Introduction - Although vaccination recommendations apply to healthcare professionals in France, there are no national vaccination coverage (VC) data for this population. We conducted a survey to measure vaccination coverage among physicians, nurses, nursing assistants, and midwives practicing in healthcare facilities in metropolitan France. Methods - A cross-sectional survey using a two-stage, stratified random sampling design. In the first stage, 35 facilities were selected from five strata defined by facility type. In the second stage, 1,127 individuals were selected across four professions and three sectors of activity. Data collection was conducted via face-to-face interviews, supplemented by data collected from the occupational physician. Results - From April to October 2009, 183 physicians, 110 nurses, 58 midwives, and 101 nursing assistants were included. Vaccination coverage for hepatitis B was 91.7%, with no significant difference between surgical staff and those in other sectors on the one hand, and between surgeons and anesthesiologists compared to other physicians on the other. For other mandatory vaccinations, coverage was 95.5% for the DTP booster and 94.9% for BCG. For recommended vaccinations, coverage was 11.4% for the 10-year DTP booster including the pertussis component, 49.7% for at least one dose of the measles vaccine, 29.9% for varicella, and 25.6% for influenza. Younger healthcare workers were better vaccinated against pertussis and measles, while those working in pediatrics and maternity care were better vaccinated against influenza and pertussis. Discussion and Conclusion - Healthcare workers are generally well covered by mandatory vaccinations but very poorly covered by recommended vaccinations. It is necessary to strengthen vaccination policies for the latter and to better document vaccination status for these vaccines across all professions. (R.A.)