Since 2006, the enterococci laboratory affiliated with the National Reference Center for Antibiotic Resistance has been receiving, on a voluntary basis, glycopeptide-resistant enterococci (GRE) strains isolated in France. These strains originate primarily from outbreaks, which are limited in duration thanks to strict hygiene measures implemented in the affected healthcare facilities. Several regions have been successively affected: the west, the center, and Paris in 2006; the east and Paris in 2007; and the east and north in 2008. Most often, the species involved is E. faecium carrying the vanA resistance gene, although the recent emergence of outbreaks caused by E. faecium vanB has been observed. Pulsed-field gel electrophoresis revealed clonal diversity between hospitals and, at times, within the same facility. Multilocus sequence typing (MLST) analysis of a representative from each clone confirmed their membership in Clonal Complex 17, which comprises ERG strains adapted to the hospital environment. The French strains also share these characteristics: resistance to ampicillin and fluoroquinolones, increased association with high-level gentamicin resistance (59.6% of strains), and more frequent presence of virulence genes (esp and hyl). (R.A.)