Objectives: A survey was carried out in 2010 in order to assess the immunization coverage among children and adolescents living in Mayotte, a French collectivity located in the South Indian Ocean. Methods: This is a transversal two-stage cluster survey. Data was collected for 676 randomly selected children aged 24 to 59 months, 692 children aged 7 to 10 years and 590 adolescents aged 14 to 15 years based on immunization cards. Results: The immunization coverage among children aged 24 to 59 months was high (close to 95%), with the exception of Hib (90%). For children aged 7 to 10 years, the immunization coverage was inadequate (d 82%) except for hepatitis B and BCG (>90% coverage). For adolescents aged 14 and 15, coverage rates were low (d 75%) and far below the target levels. Conclusion: We recommend a better monitoring of the vaccination status including a follow up and a catch-up vaccina-tion among children and teenagers.