Objectives: To assess knowledge of HIV pre-exposure prophylaxis (PrEP) and the acceptability of this new prevention method among African and Caribbean individuals encountered through prevention initiatives in the Île-de-France region. Method: A qualitative exploratory study was conducted within two organizations involved in HIV/AIDS prevention, using participant observation and focus groups. Awareness-raising initiatives targeting African and Caribbean populations served as key sites for assessing the issues and challenges of prevention among these populations and addressing knowledge and acceptability of PrEP. Results: The individuals encountered, who were from endemic countries, were well-informed about HIV prevention but had, for the most part, not yet heard of PrEP. Once explained, PrEP was considered an acceptable new prevention strategy. Informants identified two situations in particular where PrEP would offer added value to current prevention efforts: for men with multiple partners and for women who suspect their partner of infidelity. Conclusion: PrEP appears acceptable among Africans and Caribbean people in the Île-de-France region when combined with other prevention strategies, including condoms and testing. Awareness-raising efforts regarding PrEP should take into account the concerns raised by this population and contribute to a comprehensive and coordinated approach to sexual health and health promotion.