In 2008, an acute hepatitis C virus (HCV) infection was diagnosed during the pre-transplant evaluation of a 29-year-old female patient with cystic fibrosis. An epidemiological investigation was conducted to identify potential risk factors for transmission. The investigation found no evidence to suggest HCV transmission within the facility. One of the three healthcare workers who provided care at the patient’s home was HCV-positive; this serological status was unknown prior to screening in July 2009. Genetic and phylogenetic analyses of the HCV strains from the patient and the caregiver showed that the two strains were closely related. These analyses do not in any way allow for a definitive determination of the direction of transmission. An assessment of home care practices remains necessary to evaluate the risk faced by individuals cared for by an HCV-positive professional. (R.A.)