Occupation-exposure matrices are tools for assessing occupational exposures used in occupational risk epidemiology. They make it possible to automatically assign workers—based solely on their occupations (the type of job they hold within a specific industry)—a probability of being exposed to risk factors present in the workplace. This guide aims to present the pesticide occupation-exposure matrix developed as part of the Matgéné program. The pesticides assessed in this job-exposure matrix are: first, plant protection products (PPPs) used to protect plants from pests, which are grouped into four subcategories (herbicides, insecticides, fungicides, and others); and second, biocidal products used to protect humans and animals from pests, defined according to three categories: biocidal products (22 subcategories defining types of biocides), medicines with biocidal properties (2 subcategories for human medicines and veterinary medicines), and products used to disinfect medical devices. The pesticide occupation-exposure matrix was developed by experts following extensive research to document the various occupational exposure scenarios for all workers in France. The matrix provides an exposure probability (representing the percentage of workers in a given occupation deemed to be exposed) for each pesticide subcategory and category, as well as an overall assessment for all pesticides across all occupations deemed to be exposed.