The Occupational Health Division of Santé Publique France has launched the Matphyto project, which is developing a method for the retrospective assessment of occupational exposure to plant protection products in agriculture by creating crop-exposure matrices (CEMs) (Spinosi, 2008). These MCE databases provide exposure indicators for the various types of pesticides involved, based on a specific crop or group of crops. Each MCE provides a historical list of the chemical families and/or active ingredients (AI) used over the past 50 years, accompanied by three exposure indicators: probability, frequency, and intensity of use. Initially developed in mainland France, Matphyto is now implemented in three French overseas departments, notably in Guadeloupe and Martinique (Gentil, 2015). Due to its socio-economic significance, banana cultivation was the focus of the first Matphyto MCE developed in the French West Indies. This technical guide describes the banana MCE, which applies exclusively to dessert bananas for export from the French West Indies (Guadeloupe and Martinique). It outlines the agricultural characteristics of banana production in the French West Indies and the associated plant protection practices. The results show that 62 active ingredients across 29 chemical families were applied in banana production in the French West Indies from 1960 to 2015. Fungicides have been used by 100% of banana growers since the 1970s, with an average frequency over time of approximately 8 treatments per year. The six most widely used fungicide active ingredients (at least 100% usage over a period within the last 40 years) belong to the chemical families of triazoles (difenoconazole, flusilazole, and propiconazole), benzimidazoles (benomyl), imidazoles (imazalil), and morpholines (tridemorph). Herbicides have also been widely used by nearly 95% of growers since the 1970s, with an average frequency over time of about 5 treatments per year. The five most widely used herbicides (with usage rates between 70% and 90% over at least one period in the last 40 years) belong to the chemical families of aminophosphonates (ammonium glufosinate), aminophosphonates (glyphosate), quaternary ammonium compounds (diquat and paraquat), and triazines (ametryn). Insecticides were used by more than 90% of farmers between the 1960s and 1990s. Their use then steadily declined, affecting approximately 50% of farmers. Treatment frequencies have varied significantly over time, with nearly 4 treatments in the 1970s and fewer than 0.5 treatments in 2015. The 8 most widely used pesticides (with usage rates between 50% and 90% at least during one period over the past 40 years) belong to the chemical families of organophosphates (ethoprophos, fosthiazate, isazophos, and fenamiphos), organochlorines (chlordecone, HCH, and dibromochloropropane), and carbamates (oxamyl). The banana crop-exposure matrix for the French West Indies presented in this technical document was used for studies that led to the publication of a review and a report (Spinosi, 2018; Gentil, 2018).