Introduction - The Free Centers for Information, Screening, and Diagnosis (CeGIDD) were established in France in 2016, with expanded mandates in the area of sexual health. This article presents the screening activities for HIV, hepatitis B (HBV) and C (HCV), and other sexually transmitted infections (STIs), as well as the vaccination activities conducted by the CeGIDDs in 2018. It also shows how these activities have evolved since 2016. Methodology - Each year, the CeGIDDs submit an activity and performance report (RAP) to the regional health agency (ARS), which is then centralized by the General Directorate of Health (DGS). Data analyzed included the number of medical consultations, the profile of patients, screening and diagnostic activities for HIV, HBV, HCV, Chlamydia trachomatis (CT), gonorrhea, and human papillomavirus, and syphilis (number of tests performed by sex and number of positive tests by sex), as well as vaccination activity against hepatitis B and human papillomavirus. Results - In 2018, the number of medical consultations conducted, reported by 279 of the 317 existing CeGIDDs, totaled 746,000. Among those seeking care, 59.0% were men, 40.8% were women, and 0.2% were transgender individuals. Nearly two-thirds (64.3%) were women under 25 or men under 30, a proportion that has increased since 2016 (54.1%). In 2018, positivity rates were: 6.72% for CT infections, 2.92% for gonorrhea, 1.43% for syphilis, 1.20% for HBV, 0.73% for HCV, and 0.38% for HIV. Positivity rates for CT infections, HIV, and HCV decreased significantly between 2016 and 2018, and for syphilis between 2017 and 2018. Vaccination activity against HBV and HPV increased sharply between 2016 and 2018; however, the number of vaccination courses initiated remains lower than the number of vaccination indications. Conclusion - CeGIDDs serve individuals at higher risk for HIV, HBV, and other STIs, as evidenced by positivity rates higher than those found in the general population. The 2016–2018 data nevertheless show a decrease in these positivity rates for several STIs, including HIV, likely due to an increase in the proportion of young patients.