Introduction - Rotaviruses are the leading cause of acute gastroenteritis in children. In the context of vaccination, careful prospective surveillance is required to monitor and characterize rotavirus infections and detect the emergence of strains that could pose an epidemic risk. Materials and Methods - From 2006 to 2009, stool samples were collected from children with diarrhea admitted to the pediatric emergency departments of 13 university hospitals. Rotaviruses were detected and then genotyped by RT-PCR based on their VP4 and VP7 capsid proteins. Results - Of 1,947 genotyped rotaviruses, G1 (61.7%) and G9 (27.4%) strains were predominant and stable, followed by G2 (6.5%), G3 (4.0%), and G4 (2.5%), predominantly associated with P[8] (92.9%). In total, 31 atypical and reassortant strains with potential zoonotic origins were detected, notably G12 and G8 strains, some of which were genetically closely related to bovine strains. Conclusions - The genotypic stability of the rotaviruses currently circulating in France should allow for the consideration of vaccine efficacy in the short and medium term. Nevertheless, G12 and G8 strains, which are likely to emerge in the future, should be taken into account in the development of future vaccines. Surveillance of rotavirus infections should be continued to monitor the emergence of new reassortants that may not respond to current vaccines. (R.A.)