In 2017, the French National Authority for Health (HAS) reassessed its strategy for screening for the human immunodeficiency virus (HIV), and in 2018, the Joint United Nations Programme on HIV/AIDS (UNAIDS) set a target to screen 95% of people living with HIV by 2030. The HAS recommends a targeted population approach and assigns general practitioners (GPs) a key role in its implementation. There is therefore a need to facilitate HIV testing by GPs and reduce missed opportunities. A 10-month pilot study conducted in 2020 evaluated the impact of displaying a pop-up—reminding users of the targeted testing frequencies recommended by the HAS—within a prescription support software system, among a panel of 2,000 GPs. The pop-up appeared if there was a history of sexually transmitted infection and/or hepatitis C and/or tuberculosis in the past 12 months, in the absence of recorded serology or an HIV diagnosis. The impact was measured by comparing the prospective follow-up of consultations conducted during the "pilot" in 2020 with the retrospective follow-up of consultations conducted during the "pre-pilot" period in 2019. The results showed a significant increase in HIV serology orders during the pilot despite the COVID-19 pandemic. Difficulties in objectively identifying the patient profiles to target and in establishing regular HIV screening follow-ups were also revealed. This tool represents an additional means of facilitating the ordering of HIV screening by primary care physicians.