Three autochthonous cases of Zika virus occurred in southern France in August 2019. Diagnosis relied on serology and transcription-mediated amplification. Attempts for virus isolation and ZIKV genome RT-PCR detection remained negative. Since the index case was not identified, we addressed the issue of genotyping and geographical origin by performing hemi-nested PCR and sequencing in the Pr gene. Analysis of 16 genotype-specific Single Nucleotides Polymorphisms identified the Asian genotype and suggested a Southeast Asia origin.
Auteur : Durand Guillaume A, Piorkowski Géraldine, Thirion Laurence, Ninove Laetitia, Giron Sandra, Zandotti Christine, Denis Jessica, Badaut Cyril, Failloux Anna-Bella, Grard Gilda, Leparc-Goffart Isabelle, de Lamballerie Xavier
Viruses, 2020, vol. 12, n°. 3, p. 1-5
